Detection of herpesviral hematopoietic necrosis virus (Cyprinid herpesvirus 2, CyHV-2) from goldfish, Carassius auratus (L.) in Korea

In April and June 2014, mortalities of goldfish occurred at Korea. The principal signs included pale gills, severely enlarged softened spleen kidney red liver. Moreover, the histopathological characteristics observed mainly in hematopoietic tissues kidney, gill lamellae intestine. predominant changes were severe necrosis tissue. addition, nucleus exhibiting peripherally displaced chromatin particularly abundant affected fish. histological examination led to hypothesize implication a virus mortality. herpes-virus (Cyprinid herpesvirus 2, CyHV-2) DNA polymerase gene successfully detected extracted from fish using PCR assay showed 100% identity with already deposited CyHV-2 NCBI. Artificial infection trials filtrates gave cumulative 30% for injected goldfish. present study, was identified as causative pathogen epizootic